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Flow cytometric analysis of stemline heterogeneity
1Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität Münster, FRG.
Pathology, Research and Practice
|December 1, 1988
Summary
Tumor stemline heterogeneity and aneuploidy are linked, measurable by chromosome analysis or DNA cytophotometry. Flow cytometry offers advantages for analyzing DNA stemlines in malignant neoplasms.
Area of Science:
- Oncology
- Cytometry
- Genetics
Background:
- Malignant tumor stemline heterogeneity is closely linked to tumor aneuploidy.
- Both stemline heterogeneity and aneuploidy can be characterized using chromosome analysis or DNA cytophotometry.
Purpose of the Study:
- To present the advantages and drawbacks of single cell photometry versus flow cytometry for DNA analysis.
- To discuss preliminary results of flow cytometric DNA analysis in malignant neoplasms.
- To consider multiparametric measurement possibilities for quantitative analysis of biochemical and antigenic properties in DNA stemlines.
Main Methods:
- DNA analysis via single cell photometry.
- DNA analysis via flow cytometry.
- Multiparametric measurement for quantitative analysis of biochemical and antigenic properties.
Main Results:
- Preliminary results of flow cytometric DNA analysis in malignant neoplasms were discussed.
- Advantages and drawbacks of single cell photometry and flow cytometry were presented.
Conclusions:
- Flow cytometry is a viable method for DNA analysis in malignant neoplasms.
- Multiparametric flow cytometry holds potential for detailed stemline analysis.