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Updated: Dec 23, 2025
Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
Published on: February 24, 2023
Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and
Rui Liu1,2, Bin Xu1,2, Shengqing Yu3
1Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Abstract:
Mycoplasma synoviae (MS), which causes respiratory disease, eggshell apex abnormalities, infectious synovitis, and arthritis in avian species, has become an economically detrimental poultry pathogen in recent years. In China, the disease is characterized by infectious synovitis and arthritis. However, the mechanism by which MS causes infectious synovitis and arthritis remains unknown. Increasing evidence suggests that synovial fibroblasts (SF) play a key role in the pathogenesis of arthritis. Here, both RNA sequencing and tandem mass tag analyses are utilized to compare the response of primary chicken SF (CSF) following infection with and without MS. The host response between non-infected and infected cells was remarkably different at both the mRNA and protein levels. In total, 2,347 differentially expressed genes (DEGs) (upregulated, n = 1,137; downregulated, n = 1,210) and 221 differentially expressed proteins (DEPs) (upregulated, n = 129; downregulated, n = 92) were detected in the infected group. A correlation analysis indicated a moderate positive correlation between the mRNA and protein level changes in MS-infected CSF. At both the transcriptomic and proteomic levels, 149 DEGs were identified; 88 genes were upregulated and 61 genes were downregulated in CSF. Additionally, part of these regulated genes and their protein products were grouped into seven categories: proliferation-related and apoptosis-related factors, inflammatory mediators, proangiogenic factors, antiangiogenic factors, matrix metalloproteinases, and other arthritis-related proteins. These proteins may be involved in the pathogenesis of MS-induced arthritis in chickens. To our knowledge, this is the first integrated analysis on the mechanism of CSF-MS interactions that combined transcriptomic and proteomic technologies. In this study, many key candidate genes and their protein products related to MS-induced infectious synovitis and arthritis were identified.
Insights
Mycoplasma synoviae (MS) causes poultry arthritis. This study used transcriptomic and proteomic analyses to identify key genes and proteins involved in MS-induced infectious synovitis and arthritis in chicken synovial fibroblasts.
Area of Science:
- Veterinary Science
- Molecular Biology
- Pathogen Research
Background:
- Mycoplasma synoviae (MS) is an economically significant poultry pathogen causing respiratory disease and arthritis.
- The pathogenesis of MS-induced infectious synovitis and arthritis in chickens remains poorly understood.
- Synovial fibroblasts (SF) are implicated in arthritis development.
Purpose of the Study:
- To investigate the molecular mechanisms underlying Mycoplasma synoviae infection in chicken synovial fibroblasts (CSF).
- To identify differentially expressed genes (DEGs) and proteins (DEPs) in CSF upon MS infection.
- To correlate transcriptomic and proteomic data to understand host response to MS.
Main Methods:
- Primary chicken synovial fibroblasts (CSF) were infected with Mycoplasma synoviae (MS).
- Host cell responses were analyzed using RNA sequencing and tandem mass tag (proteomic) analyses.
- Differentially expressed genes and proteins were identified and correlated.
Main Results:
- A significant number of differentially expressed genes (2,347) and proteins (221) were identified in MS-infected CSF.
- A moderate positive correlation was observed between mRNA and protein level changes.
- 149 genes showed differential expression at both transcriptomic and proteomic levels, categorized into key functional groups.
Conclusions:
- This study provides the first integrated transcriptomic and proteomic analysis of chicken synovial fibroblast-Mycoplasma synoviae interactions.
- Identified genes and proteins, including those related to proliferation, apoptosis, inflammation, and angiogenesis, are potential key players in MS-induced arthritis.
- These findings offer insights into the pathogenesis of MS-induced infectious synovitis and arthritis in poultry.
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