Related Experiment Video
Updated: Feb 10, 2026
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
Published on: November 4, 2018
The properties of two recent isolates of cytoplasmic polyhedrosis viruses
Abstract:
Cytoplasmic polyhedrosis viruses (CPVs) were isolated from laboratory cultures of Bombyx mori and Spodoptera exempta. The electrophoretic profile of the RNA segments of both viruses showed similarities with that of type 1 CPV. However, whereas the virus isolate from B. mori appeared identical to type 1 CPV (as assessed by serology, as well as the comparative mobilities of RNA segments and structural polypeptides), the isolate from S. exempta was distinct. Three of the ten viral RNA segments did not co-run with type 1 RNA, and the structural polypeptides of polyhedra had different molecular weights. It is proposed that the S. exempta isolate be included as a new 'type' (type 12) in a classification of these viruses.
Insights
Researchers identified a new type of cytoplasmic polyhedrosis virus (CPV) in Spodoptera exempta. This distinct CPV, proposed as type 12, differs from the known type 1 CPV found in Bombyx mori.
Area of Science:
- Virology
- Insect Pathology
- Molecular Biology
Background:
- Cytoplasmic polyhedrosis viruses (CPVs) are significant pathogens affecting various insect species.
- Classification of CPVs relies on serological and molecular characteristics, including RNA profiles and polypeptide structures.
- Previous studies established distinct types of CPVs based on these characteristics.
Purpose of the Study:
- To isolate and characterize cytoplasmic polyhedrosis viruses (CPVs) from Bombyx mori and Spodoptera exempta.
- To compare the molecular and serological properties of these CPV isolates with known CPV types.
- To propose a new classification for the CPV isolate from S. exempta.
Main Methods:
- Virus isolation from laboratory cultures of B. mori and S. exempta.
- Electrophoretic analysis of viral RNA segments.
- Serological testing for viral identification.
- Comparative analysis of structural polypeptide molecular weights.
Main Results:
- CPV isolates from B. mori and S. exempta exhibited similarities in RNA electrophoretic profiles to type 1 CPV.
- The B. mori isolate was identified as identical to type 1 CPV based on serology and molecular analysis.
- The S. exempta isolate showed distinct characteristics, with three unique RNA segments and different structural polypeptide molecular weights.
Conclusions:
- The CPV isolate from B. mori is confirmed as type 1 CPV.
- The CPV isolate from S. exempta represents a distinct viral type.
- It is proposed to classify the S. exempta CPV isolate as a new type, designated type 12, expanding the current CPV classification system.
