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[Expression Level of LKB1 Gene in Patients with Acute Lymphoblastic Leukemia and Its Correlation with Illness
Feng Han1, Ting-Wen Lai2, Hao Li1
1Department of Clinical Laboratorial Examination, The First Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China.
Objective:
To evaluate the expression level of LKB1 gene in patients with acute lymphoblastic leukemia (ALL), and analyze its correlation with illness condition of patients.
Methods:
The 98 patients with leukemia in our hospital were divided into two groups according to the diagnosis of patients: ALL group (56 cases) and ANLL group (42 cases). The 50 healthy persons subjected to physical examination were selected as control group. The peripheral blood mononuclear cells (PBMNC) were separated from the venous blood samples of three groups, and the expression levels of LKB1 gene were detected by RT-PCR and were compared among three groups. Patients in ALL group were divided into two groups according to the expression level of LKB1 gene: low expression group (39 cases) and high expression group (17 cases). Follow-up 3 years, the curves of OS and DFS in two groups were gained by Kaplan-Meier analysis. The ratios of CR and recurrence were compared between two groups. The related risk factors (all P<0.05) influencing the survival of patients with ALL were confirmed by multivariate logistic analysis.
Results:
The expression level of LKB1 gene of control group was 1.56(1.38-1.74)>ALL group 1.32(1.22-1.40)>ANLL group 0.89(0.78-1.02). There were statistical differences among the three groups (all P<0.05). Compared with low expression group, the CR rate in high expression group were higher, but the recurrence rate were lower in high expression group (all P<0.05). Follow-up 3 years, the OS and DFS rates in high expression group were higher than those in low expression group (all P<0.05). There was negative relation between expression level of LKB1 gene and clinical risk level of ALL patients (r=-0.712, P=0.039). For standard risk ALL, the patients in LKB1 low expression group was significantey lower than that in high expression group; and for high risk ALL, the patients in LKB1 low expression group was significant higher than that in high expression group. Multivariate logistic analysis showed that the age (OR=2.872, P=0.020), peripheral blood immature cell count (OR=2.268, P=0.028) were independent risk factors for survival of patients with ALL, and the expression level of LKB1 gene (OR=0.426, P=0.016) is protective factor.
Conclusion:
The expression level of LKB1 gene in patients with ALL is low, moreover the more low expression level of LKB1 gene were, the more severe ill condition and more poor prognosis were.
