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Updated: Dec 23, 2025
Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
Published on: August 25, 2020
[Effect of miR-100 on Migration of Rat Bone Marrow Mesenchymal Stem Cells]
Xu Ye1, Ming-Wei Chen2, Tong Wang1
1Department of Hematology, Zhejiang Hospital, Hangzhou 310030, Zhejiang Province, China.
Objective:
To explore the effect of miR-100 on the migration of rat bone marrow mesenchymal stem cells(rBMMSC).
Methods:
The rBMMSC were isolated by density gradient centrifugation, cell surface epitopes of CD105, CD45, CD34, CD29 and CD44 were analyzed by flow cytometry. The rBMMSC were transfected with miR-100 mimic or inhibitor, then the expression of miR-100 in transfected cells was detected by real-time PCR. Migration test was used to observe the effect of miR-100 on cell migration ability. The secretion level of chemokine SDF-1 in culture supernatant of cells was quantitatively detected by ELISA.
Results:
The isolated cells were identified as BMMSC. After rBMMSC were transfected with miR-100 mimic or inhibitor, as compared with control group,the expression of miR-100 in rBMMSC significantly increased or decreased respectively. In the migration experiment, the rBMMSC migration was significantly inhibited in the miR-100 mimic group (P<0.01), while the rBMMSC migration was significantly enhanced in the miR-100 inhibitor group (P<0.01). The concentration of SDF-1 in the supernatant of the miR-100 mimic group and the miR-100 inhibitor group did not change significantly compared with the control group (P>0.05).
Conclusion:
miR-100 can significantly inhibit the migration of rBMMSC, but not significantly correlated with the SDF-1.
