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Identification of key miRNAs and genes for mouse retinal development using a linear model
Yishen Wang1, Xiao Wang1, Yukang Jiang2
1State Key Laboratory of Ophthalmology, Image Reading Center, Zhongshan Ophthalmic Center, Sun Yat‑Sen University, Guangzhou, Guangdong 510060, P.R. China.
Molecular Medicine Reports
|April 23, 2020
Summary
This study profiles microRNAs (miRNAs) in developing mouse retinas, identifying key regulators of retinal development. These findings offer insights into miRNA roles in cellular metabolism and signaling pathways crucial for eye development.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- MicroRNAs (miRNAs) are critical regulators of gene expression.
- Understanding miRNA roles is essential for comprehending biological processes like development.
Purpose of the Study:
- To create a detailed spatiotemporal miRNA expression profile in the developing mouse retina.
- To identify key microRNAs (miRNAs) involved in mouse retinal development.
- To investigate the mechanisms and functions of these identified miRNAs.
Main Methods:
- Utilized Locked Nucleic Acid microarrays for miRNA expression profiling.
- Employed a linear model in Python 3.6 for identifying differentially expressed miRNAs (DE-miRNAs).
- Validated findings through bioinformatics analysis and reverse transcription-quantitative polymerase chain reaction.
Main Results:
- Identified 8 key DE-miRNAs (miR-9-5p, miR-130a-3p, miR-92a-3p, miR-20a-5p, miR-93-5p, miR-9-3p, miR-709, miR-124) with low variability during mouse retinal development.
- Gene Ontology analysis revealed target genes enriched in cellular metabolic processes.
- Kyoto Encyclopedia of Genes and Genomes analysis highlighted enrichment in PI3K/AKT/mTOR, FOXO, MAPK, neurotrophin, TGF-β signaling, focal adhesion, and axon guidance pathways.
Conclusions:
- The study identified key DE-miRNAs and their target genes/pathways crucial for mouse retinal development.
- These findings provide a foundation for future research into miRNA-mediated mechanisms in eye development.
- The identified miRNAs and pathways offer potential targets for understanding and potentially treating retinal developmental disorders.
