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Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Updated: Dec 23, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Circulating miRNA 887 is differentially expressed in ARDS and modulates endothelial function.

Andrew J Goodwin1, Pengfei Li2, Perry V Halushka3,4

  • 1Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|April 24, 2020
PubMed
Summary
This summary is machine-generated.

Elevated circulating miR-887-3p levels in sepsis patients are linked to acute respiratory distress syndrome (ARDS). This microRNA influences endothelial cells, potentially contributing to ARDS development and offering a new therapeutic target.

Keywords:
acute respiratory distress syndromechemokinesleukocytesmicroRNAssepsis

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Area of Science:

  • Molecular Biology
  • Critical Care Medicine
  • Immunology

Background:

  • Circulating microRNAs (miRNAs) are implicated in cellular communication and disease.
  • The role of miRNAs in the predisposition to acute respiratory distress syndrome (ARDS) remains unclear.
  • Sepsis is a leading cause of ARDS in intensive care units.

Purpose of the Study:

  • To identify circulating miRNAs associated with sepsis-related ARDS development.
  • To investigate the impact of identified miRNAs on endothelial cell gene expression and function.
  • To explore potential therapeutic targets for ARDS.

Main Methods:

  • Plasma miRNA levels were measured in septic patients during intensive care unit admission.
  • A differentially expressed miRNA was transfected into human pulmonary microvascular endothelial cells (HPMECs).
  • Gene expression (RNA sequencing), pathway analysis, cytokine release, and leukocyte migration assays were performed.

Main Results:

  • Circulating miR-887-3p levels were significantly higher in septic patients who developed ARDS.
  • miR-887-3p transfection into HPMECs upregulated ARDS-associated genes (e.g., CXCL10, VCAM1, CASP1) and activated infection-response pathways.
  • miR-887-3p enhanced chemokine release from endothelial cells and promoted leukocyte migration.

Conclusions:

  • Circulating miR-887-3p is a potential biomarker for ARDS in critically ill sepsis patients.
  • In vitro, miR-887-3p modulates gene expression relevant to ARDS pathogenesis and neutrophil recruitment.
  • miR-887-3p may play a role in ARDS development and represents a novel therapeutic avenue.