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Using Herbal dyes as an alternative staining method for sperm evaluation.

Mohammadreza Ebrahimi1, Abbas Parham1,2

  • 1Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

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|April 24, 2020
PubMed
Summary
This summary is machine-generated.

Natural dyes from black mulberry and safflower can stain bull sperm for morphology assessment. These eco-friendly options show promise as alternatives to costly chemical stains, though they do not assess sperm viability.

Keywords:
black mulberrybullhennanatural dyesafflowerspermstaining

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Area of Science:

  • Reproductive Biology
  • Sperm Morphology Analysis
  • Natural Product Chemistry

Background:

  • Sperm staining is crucial for evaluating sperm abnormalities, fertilization potential, and cryopreservation suitability.
  • Conventional synthetic dyes pose environmental risks due to their chemical composition.
  • There is a need for sustainable and eco-friendly alternatives for sperm staining.

Purpose of the Study:

  • To investigate the potential of natural dyes from black mulberry (BM), henna (HA), and safflower (SA) as alternatives for sperm staining.
  • To evaluate the effect of these natural dyes on sperm viability and morphological parameters.
  • To compare the efficacy of natural dyes with a standard eosin-nigrosine stain.

Main Methods:

  • Bull frozen semen was prepared and stained using aqueous extracts of BM, HA, and SA, alongside eosin-nigrosine as a control.
  • Sperm viability was assessed.
  • Morphological parameters including head area (HR), head abnormality (HB), and tail abnormality (TA) were evaluated.

Main Results:

  • None of the natural dyes effectively determined sperm viability.
  • Safflower (SA) and black mulberry (BM) dyes induced distinct coloration (yellow and red, respectively) in sperm cells.
  • SA and BM stains resulted in favorable morphological assessments (e.g., SA: HR 26.55 µm, HB 0%, TA 28%; BM: HR 25.07 µm, HB 2%, TA 3%) compared to the control (HR 34.29 µm, HB 4%, TA 4%). Henna (HA) showed poor staining efficacy.

Conclusions:

  • Safflower and black mulberry dyes show potential for staining spermatozoa and assessing sperm morphology.
  • These natural dyes can serve as cost-effective, environmentally friendly alternatives to synthetic stains for morphological analysis.
  • Further research and modification are needed to optimize their use, particularly for viability assessment.