Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.4K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.4K
Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

1.6K
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
1.6K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

41.4K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.4K
Assessing Body Temperature - Temporal Artery01:19

Assessing Body Temperature - Temporal Artery

924
Here is a stepwise guide to assessing the body temperature at the temporal artery using a temporal artery thermometer
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.  
Step 3: Assess the patient's...
924
Assessing Body Temperature - Axilla01:14

Assessing Body Temperature - Axilla

1.0K
Procedural Guide for Assessing Axillary Body Temperature using a Digital Thermometer:
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
1.0K
Assessing Body Temperature - Tympanic membrane01:14

Assessing Body Temperature - Tympanic membrane

1.0K
Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
Step 1: Begin by practicing good hand hygiene to prevent the transmission of microorganisms.
Step 2: Turn on the thermometer and wait until the ready sign appears on the screen to ensure accurate measurement.
Step 3: Slide the probe cover in place to prevent cross-contamination.
Step 4: Instruct the patient to tilt their head to the side for comfort and check for cerumen...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A wearable biosensing platform for continuous monitoring of inflammatory and metabolic biomarkers for real-time health tracking and personalized care.

Bioengineering & translational medicine·2026
Same author

TRACE-QUAD: A Multiplexed Electrochemical Platform for Ultrasensitive Detection of Diquat, Paraquat, Glyphosate, and Chlorpyrifos in Drinking Water.

Journal of agricultural and food chemistry·2026
Same author

Dual-cytokine profiling of inflammatory states using a rapid electroanalytical device as a point-of-care sensor platform.

Mikrochimica acta·2026
Same author

A Non-Faradaic Impedimetric Label-Free Immunosensor Integrated with PCCODE Logic for Stratified Monitoring of Post-COVID Conditions.

ACS measurement science au·2025
Same author

Development and benchtop testing of an aptamer-based sweat sensor for CRP detection.

Mikrochimica acta·2025
Same author

Duplex EIS Sensor for <i>Salmonella Typhi</i> and <i>Aflatoxin B1</i> Detection in Soil Runoff.

Biosensors·2025

Related Experiment Video

Updated: Dec 23, 2025

Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments
08:36

Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments

Published on: August 8, 2019

12.5K

SLOCK (sensor for circadian clock): passive sweat-based chronobiology tracker.

Sayali Upasham1, Shalini Prasad

  • 1Department of Bioengineering, University of Texas at Dallas, Richardson, TX-75080, USA. Shalini.Prasad@utdallas.edu.

Lab on a Chip
|April 24, 2020
PubMed
Summary

This study introduces SLOCK, a novel sweat-based sensor for tracking circadian rhythm biomarkers like cortisol and DHEA. The platform demonstrates potential for personalized chronobiology monitoring and managing circadian rhythm disorders.

More Related Videos

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device
05:32

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Published on: November 24, 2016

8.2K
Human Circadian Phenotyping and Diurnal Performance Testing in the Real World
10:16

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

8.9K

Related Experiment Videos

Last Updated: Dec 23, 2025

Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments
08:36

Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments

Published on: August 8, 2019

12.5K
A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device
05:32

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Published on: November 24, 2016

8.2K
Human Circadian Phenotyping and Diurnal Performance Testing in the Real World
10:16

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

8.9K

Area of Science:

  • Biomarker detection
  • Chronobiology
  • Wearable sensor technology

Background:

  • Circadian rhythm disorders are linked to various health issues.
  • Current methods for monitoring circadian biomarkers are often invasive or infrequent.
  • There is a need for continuous, non-invasive monitoring of key circadian hormones.

Purpose of the Study:

  • To develop and validate a novel sweat-based sensor platform (SLOCK) for simultaneous, real-time monitoring of cortisol and dehydroepiandrosterone (DHEA).
  • To assess the sensor's performance in detecting physiological concentration changes of these biomarkers in human subjects.
  • To demonstrate the potential of SLOCK for tracking chronobiology and managing circadian abnormalities.

Main Methods:

  • Development of a hybrid porosity platform utilizing affinity-based electrochemical detection for sweat analysis.
  • Evaluation of the SLOCK sensor's performance in six healthy human subjects across different demographics.
  • Validation of sensor results against Enzyme-Linked Immunosorbent Assay (ELISA).

Main Results:

  • The SLOCK sensor demonstrated high sensitivity to cortisol and DHEA within physiological ranges (8-141 ng/mL).
  • The platform accurately captured dynamic changes in biomarker concentrations, reflecting diurnal fluctuations.
  • Cortisol detection showed percentage changes of 10-22%, while DHEA detection showed 45-56% changes, confirming sensitivity to concentration shifts.

Conclusions:

  • SLOCK represents the first demonstration of a passive, self-monitoring approach for chronobiology tracking using multiple sweat biomarkers.
  • The sensor's ability to capture dynamic biomarker changes is crucial for developing personalized circadian profiles.
  • This technology holds significant potential for managing circadian abnormalities and advancing personalized health monitoring.