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Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
Published on: March 18, 2014
Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the
Shenglin Xu1, Yuan Wang1, Yukang Que1
1Department of Orthopedics, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230000, P.R. China.
Abstract:
The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis.
Insights
Cold atmospheric plasma (CAP)-activated Ringer's solution effectively inhibits osteosarcoma cell proliferation. This effect is mediated by inducing apoptosis through increased reactive oxygen species (ROS) and decreased mitochondrial membrane potential.
Area of Science:
- Biomedical Engineering
- Oncology
- Plasma Physics
Background:
- Osteosarcoma is a primary bone malignancy with limited treatment options.
- Cold atmospheric plasma (CAP) is an emerging therapeutic modality with potential anti-cancer effects.
- Understanding the molecular mechanisms of CAP in cancer treatment is crucial for clinical translation.
Purpose of the Study:
- To investigate the anti-proliferative effects of CAP-activated Ringer's solution on osteosarcoma cell lines.
- To elucidate the molecular mechanisms, including apoptosis and reactive oxygen species (ROS) generation, involved in CAP's action.
- To assess the impact of CAP-activated Ringer's solution on mitochondrial membrane potential.
Main Methods:
- Treatment of MG63 and U2OS osteosarcoma cells with CAP-activated Ringer's solution.
- Assessment of cell viability using MTT assay.
- Detection of apoptosis via Annexin-V/propidium iodide staining.
- Western blotting for apoptosis-related proteins (cytochrome c, caspase-3, PARP).
- Measurement of mitochondrial membrane potential using JC-1 dye and ROS levels.
Main Results:
- CAP-activated Ringer's solution demonstrated dose- and time-dependent inhibition of osteosarcoma cell proliferation.
- Treatment induced significant apoptosis in MG63 cells.
- Increased intracellular ROS levels and decreased mitochondrial membrane potential were observed.
- CAP-activated Ringer's solution promoted the release of cytochrome c, indicating mitochondrial pathway activation.
Conclusions:
- CAP-activated Ringer's solution effectively inhibits osteosarcoma cell proliferation and induces apoptosis.
- The underlying mechanism involves ROS-mediated mitochondrial apoptosis pathway.
- This study highlights the potential of CAP-activated Ringer's solution as a novel therapeutic strategy for osteosarcoma.
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