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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Atherosclerosis I: Introduction01:30

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Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
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Related Experiment Video

Updated: Dec 23, 2025

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart
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Single Cell RNA Sequencing in Atherosclerosis Research.

Jesse W Williams1,2, Holger Winkels3, Christopher P Durant3

  • 1From the Department of Integrative Biology and Physiology (J.W.W.), University of Minnesota Medical School, Minneapolis.

Circulation Research
|April 24, 2020
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Single cell transcriptomics reveals new cell subsets in atherosclerotic plaques, advancing cardiovascular disease research. This guide details methods for analyzing single-cell RNA sequencing and cell surface phenotypes.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cardiovascular Research

Background:

  • Single cell transcriptomics offers unprecedented resolution for studying cellular heterogeneity.
  • Atherosclerotic plaques contain diverse cell populations crucial to disease progression.
  • Previous studies often overlooked the complexity within seemingly homogenous cell types.

Purpose of the Study:

  • To provide a technical guide for single cell transcriptomics in cardiovascular disease research.
  • To detail the design, implementation, and interpretation of single cell RNA sequencing and surface phenotype analysis.
  • To highlight the impact of these technologies on understanding atherosclerosis.

Main Methods:

  • Single cell RNA sequencing (scRNA-seq) for transcriptome profiling.
  • Simultaneous measurement of surface phenotypes.
  • Computational analysis for cell type identification and lineage tracing.

Main Results:

  • Discovery of novel cell subsets within atherosclerotic plaques.
  • Refined understanding of cell type and lineage identity in cardiovascular disease.
  • Insights into the functional roles of different cell populations in atherosclerosis.

Conclusions:

  • Single cell transcriptomics is revolutionizing cardiovascular disease research.
  • These methods enable deeper insights into the molecular mechanisms of atherosclerosis.
  • The technical guide facilitates the application of single cell approaches in the field.