Related Experiment Video
Updated: Dec 23, 2025

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Whole Lotta Lipids-from HCV RNA Replication to the Mature Viral Particle
Hanna Bley1, Anja Schöbel1, Eva Herker1
1Institute of Virology, Philipps-University Marburg, 35043 Marburg, Germany.
Abstract:
Replication of the hepatitis C virus (HCV) strongly relies on various lipid metabolic processes in different steps of the viral life cycle. In general, HCV changes the cells' lipidomic profile by differentially regulating key pathways of lipid synthesis, remodeling, and utilization. In this review, we sum up the latest data mainly from the past five years, emphasizing the role of lipids in HCV RNA replication, assembly, and egress. In detail, we highlight changes in the fatty acid content as well as alterations of the membrane lipid composition during replication vesicle formation. We address the role of lipid droplets as a lipid provider during replication and as an essential hub for HCV assembly. Finally, we depict different ideas of HCV maturation and egress including lipoprotein association and potential secretory routes.
Related Concept Videos
Viruses with RNA Genomes
Retrovirus Life Cycles
Size and Structure of Viral Genomes
Viral Structure
Retroviruses
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

