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Development of Novel Magneto-Biosensor for Sulfapyridine Detection
Talha Jamshaid1,2,3, Ernandes Taveira Tenório-Neto1,4, Abdellatif Baraket1
1Univ Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
Biosensors
|April 25, 2020
Summary
We developed a highly sensitive biosensor for sulfapyridine detection using a novel Bio-MEMS platform. Magnetic latex nanoparticles significantly enhanced the biosensor
Area of Science:
- Electrochemistry
- Biosensor Technology
- Microelectromechanical Systems (MEMS)
Background:
- Sulfapyridine is an important sulfonamide antibiotic.
- Accurate detection of sulfapyridine is crucial for food safety and clinical diagnostics.
- Existing detection methods often lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a highly sensitive and reliable biosensor for sulfapyridine detection.
- To investigate the use of Bio-MEMS and magnetic latex nanoparticles for enhanced sensitivity.
- To optimize a competitive immunoassay format for sulfapyridine quantification.
Main Methods:
- Fabrication of a Bio-MEMS platform with gold working electrodes.
- Modification of electrodes with 4-aminophenylacetic acid and immobilization of SA2BSA.
- Development of a competitive assay using Ab155 antibody immobilized on magnetic latex nanoparticles (Ab-MLNp) and electrochemical impedance spectroscopy (EIS).
Main Results:
- The developed biosensor demonstrated high sensitivity for sulfapyridine detection.
- The integration of Ab-MLNp created a 3D architecture, significantly improving assay sensitivity.
- Electrochemical impedance spectroscopy (EIS) showed increased linearity and sensitivity with the use of Ab-MLNp.
Conclusions:
- The novel Bio-MEMS based biosensor offers a highly sensitive platform for sulfapyridine detection.
- Magnetic latex nanoparticles are effective in enhancing biosensor sensitivity through a 3D architecture.
- This approach provides a promising method for rapid and accurate sulfapyridine quantification.
