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Clonal characterisation of Streptococcus pneumoniae strains using MLST and MLVA - Can MLVA improve the

J Kozáková, Z Okonji, M Musílek

    Epidemiologie, Mikrobiologie, Imunologie : Casopis Spolecnosti Pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti J.E. Purkyne
    |April 25, 2020
    PubMed
    Abstract

    Insights

    Multilocus sequence typing (MLST) confirmed clonal characteristics of Streptococcus pneumoniae causing invasive pneumococcal disease in the Czech Republic. Multiple-locus variable number tandem repeat analysis (MLVA) showed higher discriminatory power but MLST remains the standard for surveillance.

    Area of Science:

    • Microbiology
    • Epidemiology
    • Genetics

    Background:

    • Invasive pneumococcal disease (IPD) surveillance is crucial for public health.
    • Understanding the clonal characteristics of Streptococcus pneumoniae (S. pneumoniae) aids in tracking disease spread and vaccine effectiveness.
    • Standardized methods are essential for reliable epidemiological data.

    Purpose of the Study:

    • To determine the clonal characteristics of S. pneumoniae strains causing IPD in the Czech Republic in 2017.
    • To compare the utility of multilocus sequence typing (MLST) and multiple-locus variable number tandem repeat analysis (MLVA) for S. pneumoniae clonal assignment.
    • To assess the genetic variability within different S. pneumoniae serotypes.

    Main Methods:

    • Utilized 87 S. pneumoniae isolates from the Czech Republic's IPD surveillance program in 2017.
    • Employed multilocus sequence typing (MLST), a global standard for bacterial isolate characterization.
    • Applied multiple-locus variable number tandem repeat analysis (MLVA) as a newly introduced method for clonal analysis.

    Main Results:

    • MLST revealed clonal homogeneity for serotypes 1, 9N, 9V, and 22F, and genetic variability for serotypes 4 and 8.
    • A general correlation was observed between MLST and MLVA clonal complex assignments.
    • MLVA demonstrated superior clonal discriminatory power compared to MLST, identifying new MLVA types (MT).

    Conclusions:

    • MLST remains the standard method for S. pneumoniae clonal characterization in national and international IPD surveillance.
    • MLVA can serve as a complementary method for rapid identification of clonal relatedness, particularly in local outbreaks.
    • MLVA is more suitable for detecting the emergence and spread of virulent clonal variants.
    Keywords:
    MLSTMLVAStreptococcus pneumoniaeclonal characterisationserotypesurveillance

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