Related Experiment Video
Updated: Jul 17, 2026

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
Published on: July 6, 2010
A fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI
Kohei Miyazaki1, Ken-Ichi Yano2, Hisato Saitoh1,3
1Department of Biological Science, Graduate School of Science and Technology, Kumamoto University , Kumamoto, Japan.
Abstract:
Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.
Abbreviations:
DiI: 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; DiO: 3,3'-dioctadecyloxacarbocyanine perchlorate; NE: nuclear envelope; RanBP2: Ran-binding protein 2/Nucleoporin 358.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Immunofluorescence Microscopy
The...

