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Related Experiment Video

Updated: Dec 23, 2025

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

Published on: March 27, 2016

Influenza A virus uncoating.

Yohei Yamauchi¹

¹School of Cellular & Molecular Medicine, University of Bristol, Bristol, United Kingdom.

Advances in Virus Research

|April 25, 2020

Summary

This summary is machine-generated.

Histone deacetylase 6 (HDAC6) and karyopherin-β2 (kapβ2) are host proteins that uncoat influenza A virus (IAV). These cellular proteins are key to disaggregating viral components for replication, offering potential therapeutic targets.

Keywords:

Aggresome Cellular condensates Disaggregation HDAC6 Influenza A virus Karyopherin-β2 Phase separation Stress granule Ubiquitin Virus uncoating

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Last Updated: Dec 23, 2025

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

Published on: March 27, 2016

In vitro Uncoating of HIV-1 Cores

In vitro Uncoating of HIV-1 Cores

Published on: November 8, 2011

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Published on: June 3, 2012

Area of Science:

Virology
Cell Biology
Molecular Biology

Background:

Influenza A virus (IAV) entry involves sequential steps in endosomes and cytosol.
Host proteins are crucial for viral core uncoating and genome replication.
Cellular protein management machinery is increasingly recognized in viral processes.

Purpose of the Study:

To identify host factors involved in influenza virus uncoating.
To explore the role of histone deacetylase 6 (HDAC6) and karyopherin-β2 (kapβ2) in viral uncoating.
To understand the implications of cellular condensate management in viral pathogenesis.

Main Methods:

Investigated the function of HDAC6 and kapβ2 in the context of IAV infection.
Examined the interaction of these host proteins with viral components.
Analyzed the impact of these proteins on viral replication and uncoating dynamics.

Main Results:

HDAC6 and kapβ2 were identified as host proteins responsible for uncoating influenza virus.
Kapβ2 acts as a universal uncoating factor for IAV and HIV-1.
Kapβ2 can reverse liquid-liquid phase separation (LLPS) of RNA-binding proteins.

Conclusions:

Host proteins managing cellular condensates are potent viral uncoating factors.
Targeting universal virus uncoating pathways presents a promising therapeutic strategy for enveloped RNA viruses.
Understanding these host-virus interactions can inform novel antiviral therapies.