REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma
Zhiqiang Yin1, Hao Jin2, Shibo Huang3
1Department of Orthopedics, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
Background:
Osteosarcoma (OS) is the most common malignant bone tumor with high mortality in children and adolescents. REG γ is overexpressed and plays oncogenic roles in various types of human cancers. However, the expression and potential roles of REG γ in osteosarcoma are elusive. This study aims at exploring possible biological functions of REG γ in the pathogenesis of osteosarcoma and its underlying mechanism.
Methods:
Quantitativereverse transcription-polymerase chain reaction (qRT-PCR), western blotting andimmunohistochemistry (IHC)were performed to detect the expression levels of REG γ in OS tissues and cell lines. Then, the effects of REG γ expression on OS cell proliferation in vitro were analyzed by Cell Counting Kit-8 (CCK-8), ethylene deoxyuridine (EdU), colony formation, flow cytometry. The protein levels of apoptosis and cell-cycle related proteins were evaluated using western blotting.
Results:
In present study, we found for the first time that REG γ is overexpressed in osteosarcoma tissues and cell lines and knockdown of REG γ significantly inhibits cell proliferation and induces apoptosis and cell cycle arrest in osteosarcoma cells. Furthermore, we observed that p21, caspase-3 and cleaved caspase-3 are increased while the expression of cycinD1 and bcl-2 are decreased after REG γ depletion in osteosarcoma cells. In conclusion, REG γ may be involved in the proliferation of osteosarcoma and serve as a novel therapeutic target in patients with osteosarcoma.
Insights
REG γ is overexpressed in osteosarcoma, driving cancer growth. Inhibiting REG γ halts osteosarcoma cell proliferation and induces apoptosis, suggesting it as a potential therapeutic target for this bone cancer.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Osteosarcoma (OS) is a prevalent bone cancer in children and adolescents with high mortality.
- REG γ is implicated in various cancers, but its role in osteosarcoma remains unclear.
Purpose of the Study:
- To investigate the biological functions and underlying mechanisms of REG γ in osteosarcoma pathogenesis.
- To explore REG γ as a potential therapeutic target for osteosarcoma.
Main Methods:
- Expression analysis using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry (IHC).
- Functional assays including Cell Counting Kit-8 (CCK-8), EdU, colony formation, and flow cytometry to assess proliferation, apoptosis, and cell cycle.
- Western blotting to evaluate apoptosis and cell-cycle related proteins.
Main Results:
- REG γ is significantly overexpressed in osteosarcoma tissues and cell lines.
- Knockdown of REG γ inhibited osteosarcoma cell proliferation, induced apoptosis, and caused cell cycle arrest.
- REG γ depletion increased p21, caspase-3, and cleaved caspase-3, while decreasing cyclin D1 and bcl-2.
Conclusions:
- REG γ plays a crucial role in osteosarcoma proliferation and survival.
- REG γ represents a promising novel therapeutic target for osteosarcoma treatment.
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