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Related Concept Videos

Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

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Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
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Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

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Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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Current Sampling Methods for Gut Microbiota: A Call for More Precise Devices.

Qiang Tang1,2, Ge Jin1,2, Gang Wang2

  • 1Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China.

Frontiers in Cellular and Infection Microbiology
|April 25, 2020
PubMed
Summary
This summary is machine-generated.

Understanding the gut microbiome is advancing with new sequencing tech. However, current sampling methods for gut microbiota are variable and invasive, hindering clinical applications.

Keywords:
aspiratebiopsyfecesgut microbiotasampling methods

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Area of Science:

  • Microbiology
  • Genomics
  • Gastroenterology

Background:

  • Next-generation sequencing (NGS) offers deeper insights into the gut microbiome.
  • Variability in gut microbiota study results complicates clinical diagnosis and treatment.
  • Current sampling methods have limitations, including invasiveness and representativeness.

Purpose of the Study:

  • To review current gut microbiome sampling methods.
  • To highlight the advantages and disadvantages of existing techniques.
  • To introduce novel sampling technologies for improved gut microbiome analysis.

Main Methods:

  • Review of existing literature on gut microbiome sampling.
  • Analysis of current techniques: fecal, mucosal biopsy, and intestinal fluid sampling.
  • Discussion of emerging technologies like the Brisbane Aseptic Biopsy Device and intelligent capsules.

Main Results:

  • Fecal samples are indirect indicators of gut microbiota composition.
  • Biopsies are invasive and unsuitable for healthy individuals.
  • Different gastrointestinal tract sites harbor unique microbial communities.

Conclusions:

  • There is a need for non-invasive, site-specific gut microbiome sampling methods.
  • Novel technologies show promise for more precise microbiome profiling.
  • Improved sampling is crucial for reliable clinical applications of gut microbiome research.