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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Related Experiment Video

Updated: Dec 23, 2025

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Impedimetric Aptamer-Based Biosensors: Principles and Techniques.

Peggy Reich1, John-Alexander Preuß2, Nicole Bahner3

  • 1Institute of Technical Chemistry, Leibniz Universität Hannover, Hanover, Germany. p.reich@stud.uni-hannover.de.

Advances in Biochemical Engineering/Biotechnology
|April 25, 2020
PubMed
Summary
This summary is machine-generated.

Aptamer-based impedimetric biosensors offer high-affinity detection comparable to antibodies. This review highlights recent advances in these biosensors for potential handheld and point-of-care diagnostic applications.

Keywords:
AptamerAptasensorBiosensorCapacitiveDNA origamiElectrodeFaradaicImpedanceLabel-freeNanocompositesNanotechnologyNon-faradaicRapid detectionRedox mediator

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Aptamers are in vitro selected ligands with antibody-like affinity.
  • Impedance spectroscopy provides a sensitive detection method for biosensors.
  • Aptamer-based biosensors are promising for portable diagnostics.

Purpose of the Study:

  • To review recent advancements in aptamer-based impedimetric biosensors.
  • To summarize electrochemical measurement techniques relevant to these biosensors.
  • To discuss key criteria for comparing aptamer-based biosensor performance.

Main Methods:

  • Literature review of aptamer-based impedimetric biosensor research.
  • Summary of electrochemical detection strategies.
  • Analysis of biosensor comparison criteria.

Main Results:

  • Recent innovative detection strategies for aptamer-based impedimetric biosensors are detailed.
  • The review covers advances in applying aptamers with impedance spectroscopy.
  • Key performance indicators for these biosensors are discussed.

Conclusions:

  • Aptamer-based impedimetric biosensors represent a significant advancement in diagnostic technology.
  • These biosensors show great potential for point-of-care testing and handheld devices.
  • Further research and development are crucial for optimizing their clinical utility.