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[Rapid determination of protein C activity]
F Nicham1, J F Guichaoua, G Contant
1Laboratoire SERBIO, Asnières.
Annales De Biologie Clinique
|January 1, 1988
Summary
A new diagnostic method for Protein C (PC) deficiency uses snake venom and a chromogenic substrate. This rapid, sensitive assay aids in identifying PC deficiency, crucial for preventing thromboembolic events in young patients.
Area of Science:
- Biochemistry
- Hematology
- Clinical Diagnostics
Background:
- Congenital Protein C (PC) deficiency is a significant risk factor for serious thromboembolic events, particularly in young individuals.
- Even mild decreases in PC levels (40-60%) can precipitate thrombotic episodes, underscoring the need for accurate diagnostics.
Purpose of the Study:
- To develop and validate a novel, sensitive, and rapid method for measuring Protein C activity.
- To assess the utility of this new method in diagnosing congenital and acquired PC deficiencies.
Main Methods:
- Utilized an activator from Agkistrodon C. Contortrix snake venom.
- Employed the synthetic chromogenic substrate CBS 65-25 for PC activity measurement.
- Compared results with ELISA and clotting methods.
Main Results:
- The new method demonstrated comparable results to established ELISA and clotting assays in normal individuals and those with deficiencies.
- The method showed potential for routine clinical use, adaptable to standard laboratory instruments.
- Discrepancies were noted in patients undergoing anticoagulant therapy.
Conclusions:
- The developed snake venom-based chromogenic assay is a viable and sensitive tool for Protein C activity measurement.
- This method offers a rapid and accessible approach for diagnosing Protein C deficiency, aiding in the prevention of thromboembolic events.
- The assay's adaptability suggests its potential for widespread adoption in clinical chemistry laboratories.