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HPLC analysis of 6-mercaptopurine and metabolites in extracellular body fluids
J L Rudy1, J C Argyle, N Winick
1Department of Pediatrics, Children's Medical Center of Dallas, Texas 75235.
Annals of Clinical Biochemistry
|September 1, 1988
Abstract:
A convenient HPLC assay, which allows for the simultaneous measurement in extracellular fluids of 6-mercaptopurine and four of its metabolites, 6-thioguanine, 6-mercaptopurine riboside, 6-thioxanthine and 6-thiouric acid is described. Solid phase extraction allows for the clean isolation of analytes from plasma, urine or cerebrospinal fluid. The simultaneous determination of 6-mercaptopurine and some of its major metabolites in extracellular fluids may contribute to the monitoring of patient compliance, bioavailability, and individual variation in metabolism and absorption.