CircRNA_0048211 protects postmenopausal osteoporosis through targeting miRNA-93-5p to regulate BMP2

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• Postmenopausal osteoporosis (PMOP) is a significant health concern.
• Understanding the molecular mechanisms underlying PMOP progression is crucial for developing effective treatments.

Purpose of the Study:

• To investigate the role of the circRNA_0048211/miRNA-93-5p/BMP2 regulatory loop in PMOP.
• To elucidate the impact of this loop on osteogenesis and bone health.

Main Methods:

• Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from PMOP patients and healthy controls.
• Expression levels of circRNA_0048211, miRNA-93-5p, and BMP2 were quantified.
• Dual-Luciferase reporter gene assay was used to verify interactions within the regulatory loop.
• Effects on osteogenesis markers, alkaline phosphatase (ALP) activity, and mineralization were assessed.

Main Results:

• CircRNA_0048211 and BMP2 were downregulated, while miRNA-93-5p was upregulated in PMOP patients.
• Overexpression of circRNA_0048211 enhanced osteogenesis markers, ALP activity, and mineralization.
• CircRNA_0048211 negatively regulates miRNA-93-5p, which directly targets BMP2.
• The circRNA_0048211/miRNA-93-5p/BMP2 loop was confirmed to regulate osteogenesis.

Conclusions:

• CircRNA_0048211 plays a protective role in PMOP by negatively targeting miRNA-93-5p and upregulating BMP2.
• This regulatory pathway is a key determinant of osteogenesis and bone mineralization.
• The circRNA_0048211/miRNA-93-5p/BMP2 axis represents a promising therapeutic target for PMOP.