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Generation of Local CA1 γ Oscillations by Tetanic Stimulation
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TRIC-A shapes oscillatory Ca2+ signals by interaction with STIM1/Orai1 complexes
Niroj Shrestha1, Bernadett Bacsa1, Hwei Ling Ong2
1Gottfried Schatz Research Center-Biophysics, Medical University of Graz, Graz, Austria.
Plos Biology
|April 25, 2020
Summary
Trimeric intracellular cation (TRIC) channels, specifically TRIC-A, regulate calcium (Ca2+) signals by attenuating store-operated calcium entry (SOCE). This finding suggests TRIC-A dysfunction may link to muscle disorders.
Area of Science:
- Cellular Biology
- Molecular Physiology
- Calcium Signaling
Background:
- Trimeric intracellular cation (TRIC) channels are implicated in calcium (Ca2+) release from the endoplasmic reticulum (ER).
- Their precise role in modulating Ca2+ oscillations and store-operated Ca2+ entry (SOCE) remains incompletely understood.
Purpose of the Study:
- To elucidate the role of TRIC-A in regulating Ca2+ oscillations and SOCE.
- To investigate the molecular mechanism by which TRIC-A influences Ca2+ signaling pathways.
Main Methods:
- Investigated TRIC-A's effect on ryanodine receptor 2 (RyR2) and inositol 1,4,5-triphosphate receptor (IP3R)-mediated Ca2+ signals.
- Examined TRIC-A's interaction with STIM1 and Orai1 during ER Ca2+ depletion.
- Assessed the impact of TRIC-A on Orai1-mediated Ca2+ current and SOCE.
Main Results:
- TRIC-A modulates the amplitude and frequency of Ca2+ oscillations by attenuating SOCE.
- TRIC-A delays ER Ca2+ store refilling, influencing Ca2+ oscillation patterns.
- TRIC-A interacts with STIM1 and Orai1 at ER-plasma membrane junctions, impairing STIM1/Orai1 complex assembly and reducing SOCE.
Conclusions:
- TRIC-A acts as a negative regulator of STIM1/Orai1 function, thereby controlling SOCE.
- Aberrant SOCE due to TRIC-A dysfunction may contribute to muscle disorders.
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