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Related Concept Videos

Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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RNA Stability01:53

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Dec 23, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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Recent developments of small molecules targeting RNA m6A modulators.

Jing Gu1, Jun Xu1, Qidong You1

  • 1Jiangsu Key Laboratory of Drug Design and Optimization, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

European Journal of Medicinal Chemistry
|April 25, 2020
PubMed
Summary

N6-methyladenosine (m6A) RNA regulation is crucial in mammals. This review explores m6A modulators in human diseases and their potential as therapeutic targets for medicinal chemistry.

Keywords:
EpigeneticsN(6)-methyladenosineSmall-molecule regulators

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is the most prevalent internal modification in eukaryotic messenger RNA (mRNA).
  • m6A plays a critical role in regulating the mammalian transcriptome.
  • Emerging technologies like m6A-seq have facilitated the study of m6A's functions.

Purpose of the Study:

  • To review recent advancements in understanding the role of m6A modulators in human diseases.
  • To discuss the structural characteristics of m6A modulators.
  • To highlight the potential of small-molecule regulators targeting m6A-associated proteins for disease treatment and as research tools.

Main Methods:

  • Literature review of recent studies on m6A modulators and human diseases.
  • Analysis of structural characteristics of m6A-associated proteins.
  • Medicinal chemistry perspective on small-molecule targeting of m6A pathways.

Main Results:

  • The relationship between m6A and various human diseases is increasingly being elucidated.
  • Identification and understanding of m6A modulators have advanced significantly.
  • Small molecules targeting m6A proteins show promise as therapeutic agents.

Conclusions:

  • m6A modulators are implicated in the pathogenesis of numerous human diseases.
  • Targeting m6A-associated proteins with small molecules offers a promising therapeutic strategy.
  • Further research from a medicinal chemistry standpoint can accelerate the development of m6A-based therapies.