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Related Concept Videos

Types of Step-Growth Polymers: Polyesters01:20

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The introduction of polyesters has brought major development to the textile industry. The wrinkle-free behavior of polyester blends has eliminated the need for starching and ironing clothes.
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
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Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
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Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers.

Tatiana S Demina1,2, Maria G Drozdova3, Chantal Sevrin4

  • 1Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences (ISPM RAS), 70 Profsoyuznaya str., 117393 Moscow, Russia.

Molecules (Basel, Switzerland)
|April 26, 2020
PubMed
Summary

Researchers developed self-stabilizing biodegradable microcarriers using chitosan-polyester copolymers. These novel microcarriers offer high yields and support cell growth without traditional emulsifiers.

Keywords:
chitosanfibroblastsgraft-copolymersmicrocarriersoil/water emulsionpolylactidetissue engineering

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Biodegradable microcarriers are crucial for drug delivery and tissue engineering.
  • Traditional microcarrier production often relies on emulsifiers, which can complicate purification and affect biocompatibility.
  • Chitosan-based copolymers offer tunable properties for biomaterial applications.

Purpose of the Study:

  • To develop novel, self-stabilizing biodegradable microcarriers using amphiphilic chitosan-g-polyester copolymers.
  • To achieve high microcarrier yields without the use of external emulsifiers.
  • To evaluate the performance of these microcarriers in supporting cell growth in vitro.

Main Methods:

  • Oil/water solvent evaporation technique was employed.
  • Amphiphilic chitosan-g-polyester copolymers were synthesized and used as the core material.
  • Microparticle self-stabilization kinetics were monitored during production.
  • Cell morphology and proliferation were assessed after one week of in vitro cultivation.

Main Results:

  • High yields (up to 79 wt. %) of copolymer-based microparticles were achieved without emulsifiers.
  • Microparticle self-stabilization was correlated with copolymer moiety migration to the oil/water interface.
  • The microcarriers exhibited a favorable surface/volume ratio and bioadhesive surface fragments.
  • Successful cell cultivation demonstrated good cell morphology and proliferation on the microcarriers.

Conclusions:

  • Self-stabilizing biodegradable microcarriers can be efficiently produced using chitosan-polyester copolymers via solvent evaporation.
  • The developed microcarriers demonstrate excellent yield and biocompatibility, supporting cell growth.
  • This approach offers a promising alternative to traditional emulsifier-dependent methods for microcarrier fabrication.