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Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic

Moeto Nagai1, Keita Kato1, Satoshi Soga1

  • 1Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan.

Micromachines
|April 26, 2020
PubMed
Summary

This study integrates microfluidic pumps with micronozzle arrays for high-throughput single-cell manipulation. Bidirectional electrokinetic pumps enable precise control of cellular environments for research.

Keywords:
DC biased AC electrokinetic flowbidirectional electrokinetic pumpmicronozzle-arrayparallel cell manipulation

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cellular Engineering

Background:

  • High-throughput cellular environment reconstruction is crucial for investigating cell functions.
  • Integrating micropumps with one-side-open multi-channel microdevices enhances single-cell manipulation throughput, flexibility, and accessibility.

Purpose of the Study:

  • To report the integration of a polydimethylsiloxane (PDMS) micronozzle array with bidirectional electrokinetic pumps.
  • To investigate the effects of DC bias, peak-to-peak voltage, and electrode materials (Pt/Ti, ITO) on pump performance in low conductivity solutions.
  • To demonstrate the manipulation of single HeLa cells using the integrated system.

Main Methods:

  • Fabrication of a PDMS micronozzle array.
  • Integration with bidirectional electrokinetic pumps driven by DC-biased AC voltages.
  • Utilized Pt/Ti and indium tin oxide (ITO) electrodes in an isotonic solution.
  • Controlled flow direction by adjusting the DC bias.
  • Applied DC-biased AC voltage (100 kHz, 10 Vpp, -4 V DC bias) for electroosmotic flow generation.

Main Results:

  • Achieved bidirectional flow control by altering the DC bias.
  • Successfully transported single HeLa cells into nozzle holes using the integrated pump and micronozzle array.
  • Demonstrated sufficient electroosmotic flow outside the nozzle array with specific AC voltage parameters.
  • Clarified operating conditions for DC-biased AC electrokinetic pumps in biological buffers.

Conclusions:

  • The integration of micronozzle arrays and bidirectional electrokinetic pumps offers a promising method for high-throughput cell manipulation.
  • This approach provides precise control over cellular environments, advancing in vivo cellular research.
  • The clarified operating conditions are valuable for future cell manipulation applications in biological research.