Strategy for obtaining non-native protein structures using antibody cross-reactions
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Updated: Oct 1, 2026

12:30
Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
Published on: August 17, 2015
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Biophysical Chemistry
|August 1, 1988
Abstract:
Antibodies made to short peptides or to unfolded forms of proteins are often found to cross-react with intact proteins. These cross-reactions can be used to populate non-native protein conformations, possibly including protein folding intermediates, and the structures of the non-native conformations can be characterized using amide proton exchange and two-dimensional NMR.

