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Circular dichroism of isolated ricin A- and B-chains
E J Wawrzynczak1, A F Drake, P E Thorpe
1Drug Targeting Laboratory, Imperial Cancer Research Fund Laboratories, London, U.K.
Biophysical Chemistry
|September 1, 1988
Abstract:
An analysis of the circular dichroism (CD) spectra of isolated ricin A- and B-chains revealed several bands not apparent in the spectrum of intact ricin. Arithmetic combination of the A- and B-chain spectra gave a composite spectrum resembling that of native ricin, indicating that the two chains did not undergo any major conformational change upon dissociation. The addition of lactose to the B-chain at pH 7.2 caused a slight perturbation of a tryptophan-derived negative CD band centred at 283 nm without change to the overall structure of the polypeptide.