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Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
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NMR Spectroscopy: Spin–Spin Coupling01:08

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The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
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NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

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A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
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NMR Spectrometers: Resolution and Error Correction01:14

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
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Updated: Dec 23, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
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Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning.

T Gopinath1, Daniel K Weber1, Gianluigi Veglia2,3

  • 1Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN, 55455, USA.

Journal of Biomolecular NMR
|April 26, 2020
PubMed
Summary
This summary is machine-generated.

New solid-state NMR methods using multi-receiver technology enable simultaneous detection of multiple nuclei. This significantly reduces experimental time for analyzing biological solids with Nuclear Magnetic Resonance (NMR) spectroscopy.

Keywords:
HC-DUMASHC-MAeSTOSOHC-MEIOSISMulti-acquisitionMulti-receiverPolarization optimized experiments (POE)SIM-CPSolid-state NMRUltra-fast magic angle spinning

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Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Ultrafast magic angle spinning (MAS) and 1H detection have improved solid-state NMR (ssNMR) sensitivity for biopolymers.
  • Polarization optimized experiments (POE) allow simultaneous acquisition of multi-dimensional 1H- or 13C-detected ssNMR experiments with a single receiver.

Purpose of the Study:

  • To introduce new POE subclasses (HC-DUMAS, HC-MEIOSIS, HC-MAeSTOSO) utilizing dual receiver technology.
  • To expand this approach for simultaneous detection of 1H, 13C, and 15N nuclei using three receivers.
  • To accelerate ssNMR experimental time for biological solids.

Main Methods:

  • Development of new POE subclasses: HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO.
  • Implementation of dual and triple receiver technology for simultaneous multi-nuclear detection.
  • Application of ssNMR spectroscopy for analyzing biological samples.

Main Results:

  • Demonstrated simultaneous detection of 1H and 13C nuclei using dual receivers.
  • Achieved simultaneous acquisition of 1H-, 13C-, and 15N-detected homonuclear 2D spectra using three receivers.
  • Significantly reduced overall experimental time for ssNMR.

Conclusions:

  • The combination of POE and multi-receiver technology offers a powerful approach for efficient ssNMR.
  • This advancement accelerates the study of biological solids by reducing experimental duration.
  • Future applications in structural biology and materials science are anticipated.