Related Experiment Video

Updated: Dec 23, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.9K

Long Non-coding RNA MINCR Regulates miR-876-5p/GSPT1 Axis to Aggravate Glioma Progression

Zheng Li1, Xiaoxia Xie2, Xiangyun Fan3

  • 1Department of Neurosurgery, Deyang People's Hospital, Deyang, 618000, Sichuan, China.

Neurochemical Research
|April 26, 2020
PubMed

Insights

Long non-coding RNA MINCR promotes glioma progression by sponging miR-876-5p and up-regulating GSPT1. Silencing MINCR inhibits glioma cell proliferation and migration, suggesting MINCR as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in glioma development.
  • The specific function and mechanism of lncRNA MINCR in glioma are not well understood.

Purpose of the Study:

  • To investigate the role and underlying mechanism of MINCR in glioma.
  • To explore MINCR as a potential therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • In vitro and in vivo assays to assess cellular functions.
  • Subcellular fractionation and Fluorescence in situ hybridization (FISH) for localization.
  • Luciferase reporter, RNA pull-down, and RNA immunoprecipitation (RIP) assays for mechanism investigation.

Main Results:

  • MINCR was found to be significantly up-regulated in glioma tissues.
  • Depletion of MINCR inhibited glioma cell proliferation, migration, and invasion.
  • MINCR functions as a competing endogenous RNA (ceRNA) by sponging miR-876-5p, which in turn targets GSPT1.
  • Restoring miR-876 inhibition or GSPT1 up-regulation reversed the effects of MINCR depletion.

Conclusions:

  • MINCR exacerbates glioma cell proliferation and migration through the ceRNA mechanism involving miR-876-5p and GSPT1.
  • MINCR represents a promising novel therapeutic target for glioma treatment.
Keywords:
GSPT1GliomaMINCRmiR-876-5p

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K