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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Ion-Exchange Chromatography01:09

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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Anything but Conventional Chromatography Approaches in Bioseparation.

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  • 1UCIBIO, Chemistry Department, NOVA School of Science and Technology, Caparica, 2829-516, Portugal.

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Alternative bioseparation methods beyond conventional chromatography are emerging to meet the growing demand for new biological drugs and improve accessibility. This paper explores these novel approaches and their role in downstream processing challenges.

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Separation Science
  • Biotechnology

Background:

  • Packed bed chromatography is the established bioseparation method in the biopharmaceutical industry.
  • The development of new biological drugs is increasing.
  • There is a growing need to make biopharmaceuticals more accessible.

Purpose of the Study:

  • To discuss alternative chromatography approaches beyond conventional methods.
  • To analyze current and future challenges in downstream processing.
  • To provide a perspective on the evolving landscape of bioseparation.

Main Methods:

  • Literature review and analysis of emerging trends in bioseparation.
  • Discussion of alternative chromatography techniques.
  • Exploration of challenges in downstream processing.

Main Results:

  • Conventional chromatography faces limitations with the increasing complexity and number of biologics.
  • Alternative separation techniques are being developed and investigated.
  • These novel approaches aim to address efficiency, scalability, and cost-effectiveness.

Conclusions:

  • Alternative chromatography methods are poised to play a significant role in future biopharmaceutical manufacturing.
  • Addressing downstream processing challenges is crucial for biopharmaceutical accessibility.
  • Continued innovation in bioseparation is essential for the industry.