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Updated: Dec 23, 2025
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
Phycobiliproteins from Anabaena variabilis CCC421 and its production enhancement strategies using combinatory
Dinesh Kumar Saini1, Dinesh Yadav2, Sunil Pabbi3
1Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India; Centre for Conservation and Utilisation of Blue-Green Algae (CCUBGA), Division of Microbiology, ICAR - Indian Agricultural Research Institute, New Delhi 110 012, India.
Abstract:
The production of phycobiliproteins (PBPs) from cyanobacteria represents both the industrial application and their commercial value. In this study, the capability of Anabaena variabilis CCC421 for the production of PBPs was evaluated which was further improved by optimization of selected BG-11 medium components viz. FAC, K2HPO4 and trace metals. A design matrix approach using evolutionary algorithm comprised of genetic-algorithm (GA) and fuzzy-logic-methodology (FLM), i.e., GA-Fuzzy, was used for the optimization. The maximum production of PBPs obtained with combinatory approach of GA-Fuzzy was 408.5 mg/L at an optimum combination of factors (FAC 0.153 g/L, K2HPO4 0.2 g/L and Trace metals 0.5 ml/L) which was a 2.13 fold more than the control medium. This novel approach is very useful for modulating biological processes since various nutrients and metabolites have greater influence on these processes.
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