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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
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Method to decellularize the human chorion membrane.

Laura P Frazão1, Joana Vieira-de-Castro1, Cristina Nogueira-Silva2

  • 1I3B's-Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho: 3Bs Research Group, Guimarães, Portugal,; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.

Methods in Cell Biology
|April 27, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method to decellularize the human chorion membrane (HCM), a placental tissue. This process yields a decellularized HCM (dHCM) rich in extracellular matrix proteins, suitable for tissue engineering applications.

Keywords:
Chorion membraneDecellularizationExtracellular matrixMembranesPlacenta

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • The human placenta, often considered biological waste, is a rich source of extracellular matrix (ECM) proteins.
  • The human chorion membrane (HCM), a component of the placenta, is primarily composed of various collagen types, fibronectin, and laminin.
  • The potential of HCM as a substrate for tissue engineering and regenerative medicine remains largely unexplored.

Purpose of the Study:

  • To describe, for the first time, a decellularization process specifically for the human chorion membrane (HCM) alone.
  • To evaluate the efficacy of the decellularization protocol in removing cellular components while preserving the ECM structure.
  • To prepare a decellularized human chorion membrane (dHCM) for potential applications in tissue engineering and regenerative medicine.

Main Methods:

  • Development and implementation of a novel decellularization protocol targeting the human chorion membrane (HCM).
  • Quantification and analysis of cell nuclei and double-stranded DNA (dsDNA) to verify decellularization success using DAPI staining, PicoGreen, and electrophoresis.
  • Characterization of the resulting decellularized human chorion membrane (dHCM) for its structural integrity and composition.

Main Results:

  • A successful decellularization protocol for the human chorion membrane (HCM) was established.
  • Verification methods confirmed a significant reduction in cellular nuclei and dsDNA, indicating effective decellularization.
  • The resulting decellularized human chorion membrane (dHCM) is a compact and handleable membrane, preserving its ECM structure.

Conclusions:

  • The decellularization of the human chorion membrane (HCM) alone is feasible using the described method.
  • The obtained decellularized human chorion membrane (dHCM) retains its extracellular matrix (ECM) structure.
  • This dHCM represents a promising biomaterial for future applications in tissue engineering and regenerative medicine.