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Updated: Dec 23, 2025

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Published on: July 20, 2019

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Nuclear localization of ING3 is required to suppress melanoma cell migration, invasion and angiogenesis

Ruiyao Zhou1, Anand Rotte2, Gang Li2

  • 1Department of General Surgery, The Third Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Biochemical and Biophysical Research Communications
|April 27, 2020
PubMed

Insights

The nuclear localization sequence (NLS) of the tumor suppressor ING3 was identified. ING3

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Inhibitor of growth family member 3 (ING3) is a tumor suppressor involved in cell cycle regulation, apoptosis, and transcription.
  • Nuclear ING3 has demonstrated significant biological functions, but its nuclear localization sequence (NLS) and specific roles remain undefined.

Purpose of the Study:

  • To identify the NLS of ING3.
  • To investigate the biological functions of ING3 nuclear localization in melanoma.

Main Methods:

  • Generation and analysis of various ING3 mutants to pinpoint the NLS.
  • Site-directed mutagenesis to create the ING3-K164A mutant.
  • Overexpression studies in melanoma cells to assess cellular localization and functional impact.

Main Results:

  • The NLS of ING3 was identified as the KKFK sequence between amino acids 164 and 167.
  • Mutation of Lysine 164 to Alanine (ING3-K164A) resulted in cytoplasmic retention of ING3.
  • Overexpression of wild-type ING3 inhibited melanoma cell migration, invasion, and angiogenesis, effects that were abolished by the ING3-K164A mutant.

Conclusions:

  • This study successfully identified the NLS of ING3, highlighting its critical role in nuclear transport.
  • Nuclear localization of ING3 is essential for its tumor-suppressive functions, including the inhibition of melanoma cell migration, invasion, and angiogenesis.
  • Further research is warranted to explore the post-translational modifications of ING3 at residue 164 and their impact on nuclear transport and cancer development.
Keywords:
ING3InvasionMelanomaMigrationNuclear localization sequence

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