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ID sequence-binding protein factors during development of mice.
1Department of Biochemistry, College of Science and Technology, Nihon University, Tokyo, Japan.
Brain Research
|December 1, 1988
Summary
Researchers identified a novel protein factor that binds to the ID repetitive sequence in mouse brains. This factor influences the developmental expression of small RNA transcripts from this sequence.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- The ID repetitive sequence is transcribed into small RNA in a brain-specific and developmentally regulated manner.
- Brain-specific transcription factors and RNA polymerase III are implicated in controlling gene activity during development.
Purpose of the Study:
- To investigate the proteins that bind to the ID repetitive sequence.
- To understand the regulatory mechanisms controlling the developmental expression of ID sequence-derived small RNAs.
Main Methods:
- Electrophoretic mobility shift assay (EMSA) was performed on extracts from mouse brains and livers.
- Analysis focused on identifying protein factors interacting with the promoter region of the ID sequence.
Main Results:
- A specific protein factor that binds to the region between two RNA polymerase III promoter sequences of the ID sequence was detected.
- The expression pattern of this protein factor correlates with the postnatal accumulation of small RNA transcripts from the ID sequence.
- A penta-nucleotide repeat (GCAAG) and its inverted complement (CTTGC) within the binding region may serve as the binding site for this protein factor.
Conclusions:
- A novel protein factor interacting with the ID repetitive sequence has been identified.
- This protein factor likely plays a role in the developmental regulation of small RNA synthesis from the ID sequence in the brain.
- The findings provide insights into the molecular mechanisms governing the expression of repetitive elements in a developmental context.