Related Experiment Video
Updated: Dec 23, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Genomic characterization of cycad leaf necrosis virus, the first badnavirus identified in a gymnosperm
R A Alvarez-Quinto1, B E L Lockhart2, J L Fetzer3
1Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA. alvar419@umn.edu.
Abstract:
A previously undescribed badnavirus was isolated from Zamia fischeri showing symptoms of chlorosis, necrosis, and ringspot. The virus has bacilliform virions 30 nm in diameter and averaging 120 nm in length. The viral genome is 9227 bp in length and contains three open reading frames characteristic of members of the genus Badnavirus. The largest open reading frame (ORF3) encodes a putative polyprotein, with predicted domains including zinc finger, aspartic protease, reverse transcriptase (RT) and RNase H. The virus is tentatively named "cycad leaf necrosis virus" (CLNV). Within the genus Badnavirus, CLNV was most closely related to sugarcane bacilliform Guadeloupe D virus (FJ439817), sharing 69% identity at the nucleotide level in the RT + RNase H region. This virus is the first badnavirus reported to infect cycads, and it has the largest genome among the currently characterized badnaviruses.
More Related Videos
11:12Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
Published on: April 12, 2017
07:23Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
Published on: September 14, 2021
Related Concept Videos
Viruses of Archaea
Subviral Agents
Synthetic Biology
Golden rice
Golden rice is a genetically modified...
Viral Structure
Retrovirus Life Cycles
Viruses with RNA Genomes