In situ detection of Australian gill-associated virus with a yellow head virus gene probe
Kathy F-J Tang1, Kirsten M Spann2, Leigh Owens3
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA.
Abstract:
A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.
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