Comparison effects of olive leaf extract and oleuropein compounds on male reproductive function in cyclophosphamide
Ayoob Rostamzadeh1, Hossein Amini-Khoei2, Mohammad Javad Mardani Korani2
1Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract:
Spermatogenesis is a complicated process in which sperm is susceptible to various chemotherapy drugs such as cyclophosphamide (CP). As olive leaf extract (OLE) and its active ingredient, oleuropein, have variousantioxidant, anti-apoptotic, and anti-inflammatory properties the aim of the present study was to investigate the effects of OLE and oleuropein on male reproductive function focusing antioxidative effects and histological modifications in the testes of CP-exposed mice. In order to do this, 80 NMRI male mice were divided into eight groups including control group, group received CP, group received OLE, group received oleuropein, group received OLE following CP exposure, group received oleuropein following CP exposure, group received OLE plus oleuropein and group received OLE plus oleuropein following CP exposure. In all groups CP (single dose of 100 mg/kg (, OLE (100 mg/kg for consequence 28 days) and oleuropein (100 mg/kg for consequence 28 days) were injected intraperitoneally. Moreover, testis histology, sperm parameters and serum levels of LH, FSH, MDA and antioxidant capacity were investigated. Results showed that CP caused oxidative state and abnormal changes in sperms and testes. Besides, treatments with oleuropein and OLE led to mitigate the harmful effects of CP on the male reproductive system. In conclusion, our findings showed that olive's compounds can diminish the hazardous effects of CP on spermatogenesis in mice.
Insights
Olive leaf extract (OLE) and oleuropein protect male reproductive function from chemotherapy damage. These natural compounds mitigate cyclophosphamide (CP)-induced oxidative stress and improve testicular health and sperm parameters in mice.
Area of Science:
- Reproductive Biology
- Toxicology
- Pharmacology
Background:
- Spermatogenesis is vulnerable to chemotherapy agents like cyclophosphamide (CP).
- Olive leaf extract (OLE) and its component oleuropein possess antioxidant, anti-apoptotic, and anti-inflammatory properties.
- Understanding protective effects against chemotherapy-induced reproductive toxicity is crucial.
Purpose of the Study:
- To investigate the protective effects of OLE and oleuropein against cyclophosphamide (CP)-induced testicular toxicity in male mice.
- To evaluate the impact on spermatogenesis, testicular histology, and oxidative stress markers.
- To explore the potential of olive-derived compounds in mitigating chemotherapy side effects on male fertility.
Main Methods:
- NMRI male mice were divided into eight groups, including control, CP-only, OLE-only, oleuropein-only, and combinations with CP exposure.
- Mice received intraperitoneal injections of CP (100 mg/kg), OLE (100 mg/kg), and oleuropein (100 mg/kg) for 28 days.
- Evaluated parameters included testis histology, sperm count and motility, serum LH, FSH, MDA levels, and antioxidant capacity.
Main Results:
- Cyclophosphamide (CP) exposure induced significant oxidative stress, abnormal sperm parameters, and testicular damage.
- Treatment with OLE and oleuropein ameliorated CP-induced testicular toxicity.
- Both OLE and oleuropein, individually and combined, demonstrated protective effects against CP-induced reproductive damage.
Conclusions:
- Olive leaf extract (OLE) and oleuropein possess significant protective properties against cyclophosphamide-induced male reproductive toxicity.
- These findings suggest that olive-derived compounds can mitigate the hazardous effects of chemotherapy on spermatogenesis.
- OLE and oleuropein show potential as therapeutic adjuncts to preserve male fertility during chemotherapy.
