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Published on: August 21, 2019
T-cell Epitope-based Vaccine Design for Nipah Virus by Reverse Vaccinology Approach
Praveen K P Krishnamoorthy1, Sekar Subasree1, Udhayachandran Arthi1
1Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Sriperumbudur 602117, Tamilnadu, India.
Aim And Objective:
Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spreads in humans through breathing resulting in an indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted.
Materials And Methods:
Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T - cell-based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin.
Results:
Two epitopes, SPVIAEHYY and LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol and 0.15 kcal/mol with HLA- B*35:03 and HLA- DRB1 * 07:03, respectively by using allele - Class I and Class II from AutoDock. These two peptides predicted by the reverse vaccinology approach are likely to induce immune response mediated by T - cells.
Conclusion:
Simulation using GROMACS has revealed that LVNDGLNII epitope forms a more stable complex with HLA molecule and will be useful in developing the epitope-based Nipah virus vaccine.
