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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

PCR

PCR

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Related Experiment Video

Updated: Dec 23, 2025

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

Emulsion PCR made easy.

Vaishali Verma¹, Amita Gupta^1,2, Vijay K Chaudhary^1,2

¹Centre for Innovation in Infectious Disease Research, Education & Training (CIIDRET), University of Delhi South Campus, New Delhi, 110021, India.

|April 28, 2020

Summary

This summary is machine-generated.

This study presents a streamlined protocol for emulsion PCR (ePCR) product purification. The new method simplifies DNA extraction, making ePCR accessible and safe for all labs.

Keywords:

aptamer chimerization emulsion PCR extraction next-generation sequencing silica column purification

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Last Updated: Dec 23, 2025

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

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Published on: March 9, 2021

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

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Published on: May 1, 2013

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

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Published on: September 25, 2018

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

Emulsion PCR (ePCR) amplifies DNA in droplets, preventing artifacts.
Current ePCR product recovery is cumbersome, involving hazardous solvents and multiple steps.

Purpose of the Study:

To develop a simplified, safer ePCR extraction protocol.
To improve the accessibility of ePCR for routine laboratory use.

Main Methods:

A novel protocol for direct silica-based DNA purification of ePCR products.
Elimination of hazardous organic solvent extractions.

Main Results:

Purified ePCR products are compatible with downstream applications.
The protocol significantly reduces the complexity and hazard of ePCR product recovery.

Conclusions:

The described method offers an easy, safe, and accessible approach to ePCR.
This advancement supports broader adoption of ePCR in research settings.