Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bubble Perfusion Brain Slice Culture with Single-Droplet Stimulus Delivery in a 3D Printed Microfluidic Device.

ACS measurement science au·2025
Same author

Online integration of capillary electrophoresis and dual detector Taylor dispersion analysis <i>via</i> a 3D printed instrument.

The Analyst·2025
Same author

Second harmonic scattering investigation of bacterial efflux induced by the antibiotic tetracycline.

The Journal of chemical physics·2024
Same author

Miltefosine impacts small molecule transport in Gram-positive bacteria.

RSC chemical biology·2024
Same author

A Scalable, Modular Degasser for Passive In-Line Removal of Bubbles from Biomicrofluidic Devices.

Micromachines·2023
Same author

Monitoring membranes: The exploration of biological bilayers with second harmonic generation.

Chemical physics reviews·2022
Same journal

Scalable Quantum-Classical Hybrid Algorithm for Excited States Based on Divide-and-Conquer Unitary Coupled-Cluster Linear-Response Theory Using Dynamical Polarizability.

The journal of physical chemistry. A·2026
Same journal

Donor-Acceptor Strength from the Real-Space-Partitioning of One Particle Transition Density Matrix.

The journal of physical chemistry. A·2026
Same journal

A Deterministic Computational Framework for Exact Orientational Averaging and Complete Irreducible SO(3) Decomposition of Dynamic Hyperpolarizability Tensors.

The journal of physical chemistry. A·2026
Same journal

Exploration of the van der Waals Region of the NO <i>A</i><sup>2</sup>Σ<sup>+</sup> + N<sub>2</sub> <i>X</i><sup>1</sup>Σ<sub><i>g</i></sub><sup>+</sup> Collision Complex.

The journal of physical chemistry. A·2026
Same journal

The Molecular and Electronic Structure of NdF<sub>2</sub><sup>-/0</sup>.

The journal of physical chemistry. A·2026
Same journal

The Two-Photon Polarization Ratio Explains Unusual Shape of the Two-Photon Absorption Spectra of Dyes and Detects Weak Excitonic Coupling in Fluorescent Protein Dimers.

The journal of physical chemistry. A·2026
See all related articles

Related Experiment Video

Updated: Dec 23, 2025

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K

Total Internal Reflection Transient Absorption Microscopy: An Online Detection Method for Microfluidics.

Brandon A Colon1, Muhammad Redwan Hassan1, Amirus Saleheen1

  • 1Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996, United States.

The Journal of Physical Chemistry. A
|April 28, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed online transient absorption microscopy for microfluidic reactors. This method enables real-time monitoring and optimization of chemical reactions, overcoming previous limitations in continuous flow systems.

More Related Videos

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
10:26

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

13.8K
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

3.9K

Related Experiment Videos

Last Updated: Dec 23, 2025

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K
Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
10:26

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

13.8K
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

3.9K

Area of Science:

  • Chemical Engineering
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Microreactors offer precise control over synthetic parameters for efficient production.
  • Lack of online detection and optimization methods hinders microfluidic reactor advancement.
  • Continuous flow systems require real-time monitoring for process control.

Purpose of the Study:

  • To introduce and characterize total internal reflection transient absorption microscopy (TIRTAM) for microfluidic applications.
  • To demonstrate online monitoring of excited state dynamics in continuous flow devices.
  • To provide a method for optimizing chemical reactions within microfluidic systems.

Main Methods:

  • Utilized a total internal reflection transient absorption microscopy (TIRTAM) instrument.
  • Applied TIRTAM to image excited state dynamics on a continuous flow microfluidic device.
  • Performed online transient absorption measurements.

Main Results:

  • Successfully imaged excited state dynamics in a microfluidic reactor.
  • Demonstrated the ability to discriminate between different chromophores.
  • Showcased differentiation of local chemical environment effects on chromophores.

Conclusions:

  • This work presents the first online transient absorption measurements in microfluidic devices.
  • TIRTAM provides a novel approach for real-time analysis of chemical processes.
  • This method offers a new direction for the advancement and optimization of microfluidic chemical reactions.