Related Experiment Video

Updated: Dec 23, 2025

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.9K

Elucidating the multi-drug resistance mechanism of Enterococcus faecalis V583: A gene interaction network analysis

Aniket Naha1, Sravan Kumar Miryala1, Reetika Debroy1

  • 1Medical and Biological Computing Laboratory, School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India.

Gene
|April 28, 2020
PubMed

Insights

Multi-drug resistant (MDR) bacteria pose a global threat. This study analyzes antimicrobial resistance (AMR) genes in Enterococcus faecalis V583, identifying key genes and mechanisms for potential new drug targets.

Area of Science:

  • Microbiology
  • Genetics
  • Computational Biology

Background:

  • The rise of multi-drug resistant (MDR) pathogenic bacterial strains, like Enterococcus faecalis V583, presents a significant global health challenge.
  • Understanding the specific antimicrobial resistance (AMR) genes and their mechanisms is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the acquired exogenous AMR genes in Enterococcus faecalis V583.
  • To construct and analyze an interaction network of AMR genes and their functional partners.
  • To identify potential novel drug targets for combating antibiotic resistance.

Main Methods:

  • Construction and analysis of an interaction network involving eight AMR genes and 40 functional partners.
  • Functional enrichment analysis to identify genes involved in Cellular Component, Molecular Functions, and Biological Process.
  • Clustering analysis to group genes associated with specific AMR mechanisms.

Main Results:

  • Four distinct clusters of interconnected genes (C1-C4) were identified, linked to three primary AMR mechanisms: drug target alteration, target site bypass, and ABC transporter efflux pumps.
  • Genes conferring resistance to beta-lactams, glycopeptides, and MLSB antibiotics, along with mur genes, were found to be critical in MDR.
  • Network analysis revealed hub genes (mraY, pbpC, murE, murG, murD) with a high number of interactions, indicating their potential as drug targets.

Conclusions:

  • The study elucidates the complex interplay of AMR genes in Enterococcus faecalis V583.
  • Identified hub genes represent promising targets for the development of novel antimicrobial therapies.
  • This research contributes to a deeper understanding of antibiotic resistance mechanisms and strategies for drug discovery.
Keywords:
Antibiotic therapyEnterococcus faecalisGene ontologyMulti-drug resistanceNovel targets

More Related Videos

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

964
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.7K

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
991
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
399
Antibiotic Selection00:57

Antibiotic Selection

Overview
59.2K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
6.6K
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
216