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Serial Peripheral Blood Gene Expression Profiling to Assess Immune Quiescence in Kidney Transplant Recipients with
V Ram Peddi1, Parul S Patel1, Courtney Schieve2
1Department of Transplantation, California Pacific Medical Center, San Francisco, CA, USA.
Annals of Transplantation
|April 29, 2020
Summary
TruGraf, a blood test measuring gene expression, shows high accuracy in assessing kidney transplant health. This biomarker test can help manage patients without invasive surveillance biopsies, especially in centers not performing them.
Area of Science:
- Biomarker discovery and validation
- Transplant immunology
- Genomic medicine
Background:
- TruGraf is a blood-based gene expression biomarker test.
- Surveillance biopsies are standard for kidney transplant monitoring but not universally performed.
- Evaluating TruGraf's utility in centers avoiding biopsies is crucial.
Purpose of the Study:
- To assess the clinical validity of TruGraf in stable kidney transplant recipients.
- To demonstrate the clinical utility of serial TruGraf testing.
- To support patient management in centers not using surveillance biopsies.
Main Methods:
- Collected serum creatinine, serial TruGraf results, and clinical follow-up data.
- Analyzed 28 stable kidney transplant recipients.
- Assessed concordance and negative predictive value (NPV) of TruGraf results.
Main Results:
- Overall TruGraf concordance was 77% with independent clinical assessment.
- Negative predictive value (NPV) was high at 98.0%.
- 77% of TruGraf results indicated potential usefulness in patient management.
Conclusions:
- Serial TruGraf testing is valuable for kidney transplant centers not performing surveillance biopsies.
- High NPV of TruGraf confirms immune quiescence, potentially avoiding biopsies.
- TruGraf offers a non-invasive method for monitoring transplant recipients.
