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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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DNA Microarrays02:34

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Screening of Long Noncoding RNAs Induced by Radiation Using Microarray.

Yilong Wang1, Qi Wang1, Shuangjing Chen1

  • 1Department of Radiobiology, Beijing Institute of Radiation Medicine, Beijing, China.

Dose-Response : a Publication of International Hormesis Society
|April 29, 2020
PubMed
Summary
This summary is machine-generated.

This study reveals novel long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) affected by radiation in cancer cells. These findings enhance understanding of radiation response, cell cycle regulation, and DNA damage repair mechanisms.

Keywords:
expression microarraygene ontologylncRNAradiation

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genomics

Background:

  • DNA damage repair and G2/M arrest are critical for cancer cell survival post-radiation.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in DNA repair and cell cycle regulation.
  • The genome-wide functions of lncRNAs in radiation-exposed tumor cells remain largely unexplored.

Purpose of the Study:

  • To profile genome-wide lncRNA and mRNA expression in human cancer cell lines following gamma irradiation.
  • To identify common differentially expressed lncRNAs and mRNAs across multiple cell lines.
  • To predict the functional roles of these differentially expressed lncRNAs in response to radiation.

Main Methods:

  • Utilized LncRNA + mRNA Human Gene Expression Microarray V4.0.
  • Exposed HeLa, MCF-7, and A549 cells to 4 Gy gamma radiation.
  • Performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Main Results:

  • Identified hundreds of differentially expressed lncRNAs and mRNAs in each cell line post-irradiation.
  • Found 14 common differentially expressed lncRNAs and 22 common differentially expressed mRNAs across all three cell lines.
  • Pathway analysis revealed cell cycle as a significantly affected process for differentially expressed mRNAs.

Conclusions:

  • This study provides a comprehensive catalog of radiation-responsive lncRNAs and mRNAs in cancer cells.
  • The findings highlight the involvement of specific lncRNAs in regulating cell cycle and DNA damage response pathways.
  • This research expands the understanding of lncRNA functions in the context of radiation oncology and cancer biology.