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Related Experiment Video

Updated: Dec 23, 2025

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Platform for quantitative multiscale imaging of tissue composition.

Michael A Pinkert1,2,3, Zachary J Simmons2,4, Ryan C Niemeier2,4

  • 1Morgridge Institute for Research, 330 N Orchard St, Madison, WI 53715, USA.

Biomedical Optics Express
|April 29, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel multiscale imaging system to study cellular and tissue structural changes in diseases. This integrated platform combines multiple imaging modalities for comprehensive biological analysis.

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Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Pathology

Background:

  • Multiscale structural changes in biological tissues are critical in disease pathogenesis.
  • Current limitations exist in imaging platforms capable of analyzing both cellular and tissue levels simultaneously.
  • A unified imaging system can reduce bias and simplify sample preparation for multiscale analysis.

Purpose of the Study:

  • To develop and validate a novel multiscale imaging platform.
  • To integrate diverse imaging modalities for comprehensive tissue analysis.
  • To address the need for sensitive, multiscale imaging in disease research.

Main Methods:

  • Development of a multiscale imaging system integrating Ultrasound, Second Harmonic Generation Microscopy, Multiphoton Microscopy, Optical Coherence Tomography, and Enhanced Backscattering.
  • System design and calibration for each imaging modality.
  • Demonstration of the system's capability through imaging a rabbit eye.

Main Results:

  • Successful integration of multiple imaging modalities into a single platform.
  • Demonstrated sensitivity across cellular and macrostructural spatial scales.
  • Validated system performance with a rabbit eye imaging experiment.

Conclusions:

  • The developed multiscale imaging system offers a powerful tool for investigating disease-related structural changes.
  • This integrated approach facilitates unbiased, comprehensive analysis from cellular to tissue levels.
  • The platform has the potential to advance our understanding of diseases linked to tissue structural alterations.