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The functional study of human proteins using humanized yeast.

Seho Kim1, Juhee Park1, Taekyung Kim2

  • 1Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea.

Journal of Microbiology (Seoul, Korea)
|April 29, 2020
PubMed
Summary

Humanizing yeast Saccharomyces cerevisiae by replacing its genes and nucleosomes with human counterparts enables direct study of human proteins. This approach also expands yeast

Keywords:
Saccharomyces cerevisiaebudding yeastfunctional study of proteinshuman proteinshumanization of yeast

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Optimal gene expression is vital for all life.
  • Yeast Saccharomyces cerevisiae shares conserved genes and expression mechanisms with humans, making it a valuable model organism.
  • Previous research utilized yeast to study human gene and protein functions.

Purpose of the Study:

  • To review recent studies involving the replacement of human genes and nucleosomes with yeast equivalents.
  • To highlight the utility of a 'humanized' yeast model for direct human protein research.
  • To explore the expanded applications of yeast as a model organism, including drug screening.

Main Methods:

  • Review of recent scientific literature focusing on yeast 'humanization' studies.
  • Analysis of studies where human genes and nucleosomes were replaced by yeast counterparts.
  • Examination of the implications for studying human proteins and broader biological applications.

Main Results:

  • Recent studies demonstrate successful replacement of human genetic and nucleosomal components with yeast equivalents.
  • The 'humanized' yeast model facilitates direct investigation of human proteins within a simplified eukaryotic system.
  • This approach broadens the scope of yeast as a model organism for diverse research areas.

Conclusions:

  • Humanizing yeast, despite its eukaryotic simplicity, offers a powerful platform for direct human protein studies.
  • Yeast serves as an effective model for understanding human gene expression and protein function.
  • The humanized yeast model has potential applications in drug screening and other biomedical research.