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Published on: July 25, 2011
MiR-99b regulates cerebral ischemia neuronal injury through targeting IGF1R
Dengbin Qi1, Wei Wang2, Ying Zhang3
1Department of Neurology, Affiliated Hospital of Jining Medical University, YanZhou Branch, Jining, China.
Background:
Recently, microRNA-99b (miR-99b) shows diverse functions in different human disease. However, further studies about the potential effect of miR-99b in cerebral ischemia injury still need to be done.
Methods:
The expressions of miR-99b and IGF1R were detected via RT-qPCR assay. Western blot assay was applied to measure the protein expression of Caspase-3, Bax and Bcl-2. MTT assay was used to observe cell viability of SH-SY5Y cells. The association of miR-99b and IGF1R was testified by dual luciferase assay. And human SH-SY5Y cells were treated with the oxygen-glucose deprivation/reperfusion (OGD/R) to mimic CIR injury.
Results:
The expression of miR-99b was increased in the OGD/R model. And upregulation of miR-99b promoted cell viability and inhibited apoptosis induced by OGD/R. Moreover, IGF1R was confirmed as a direct target gene of miR-99b. The expression of IGF1R was obviously decreased under OGD/R conditions.
Conclusions:
MiR-99b promoted the viability and suppressed apoptosis of SH-SY5Y cells under OGD/R conditions through targeting IGF1R.
Insights
MicroRNA-99b (miR-99b) enhances cell viability and reduces apoptosis in cerebral ischemia injury models. This protective effect is mediated by miR-99b targeting Insulin-like Growth Factor 1 Receptor (IGF1R).
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- MicroRNA-99b (miR-99b) exhibits varied roles in human diseases.
- The specific function of miR-99b in cerebral ischemia (CI) remains underexplored.
Purpose of the Study:
- To investigate the role and mechanism of miR-99b in cerebral ischemia injury.
- To determine if miR-99b affects neuronal viability and apoptosis in a CI model.
Main Methods:
- Real-time quantitative PCR (RT-qPCR) for miR-99b and IGF1R expression.
- Western blot for apoptosis-related proteins (Caspase-3, Bax, Bcl-2).
- MTT assay for cell viability and dual-luciferase assay to confirm miR-99b targeting of IGF1R in an oxygen-glucose deprivation/reperfusion (OGD/R) model of SH-SY5Y cells.
Main Results:
- miR-99b expression was upregulated in the OGD/R model.
- Increased miR-99b enhanced SH-SY5Y cell viability and inhibited OGD/R-induced apoptosis.
- IGF1R was identified as a direct target of miR-99b, with its expression decreasing under OGD/R conditions.
Conclusions:
- miR-99b plays a protective role in cerebral ischemia injury.
- The mechanism involves miR-99b targeting IGF1R, thereby promoting cell viability and suppressing apoptosis in neuronal cells under OGD/R conditions.