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Improved transfer of high molecular weight proteins using fabric-reinforced soft polyacrylamide gels
M Starita-Geribaldi1, J Giudicelli, P Sudaka
1Laboratoire de Biochimie, Faculté de Médecine Chemin de Valombrose, Nice, France.
Electrophoresis
|May 1, 1988
Abstract:
Rapid transfer of electrophoretically separated, high molecular weight proteins from fabric-reinforced, soft, low-concentration polyacrylamide gels (3.5%T, 2.6%C) is described. Polyester fabrics proved useful for gel reinforcement during vertical slab gel electrophoresis. The fabric-reinforced gel retains its dimension during transfer, equilibration and drying, facilitating comparison of protein bands in the separation gel and transfer matrix.