Changes in microRNAs Expression Profile of Mimetic Aging Mice Treated with Melanin from Sepiella japonica Ink

A natural melanin extracted from Sepiella japonica ink (MSJI) is a polymer with antioxidant properties. In this study, the effects of MSJI treatment on microRNAs differentially expressed during aging in mimetic mice were investigated. The results revealed that 8 miRNAs: mmu-miR-1971, mmu-miR-3070b-3p, mmu-miR-320-3p, mmu-miR-342-3p, mmu-miR-350-3p, mmu-miR-5132-5p, mmu-miR-697, and mmu-miR-712-5p showed significantly different expression between mice treated with MSJI gavage and aging mice. GO analysis and signaling pathway analysis revealed that the predicted target genes were involved in diverse biological processes such as steroid and cholesterol metabolism, xenobiotic, demethylation, and circadian regulation of gene expression, suggesting a potential role in antiaging. The dual-luciferase reporter gene assay confirmed the downregulation of mmu-miR-697 in HS samples and targeting of the Gpt2 which plays an important role in aging. This study supports the hypothesis that MSJI prolongs the cell cycle by acting as an antioxidant to delay decrepitude.