Related Experiment Video

Updated: Dec 23, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

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miR-4417 suppresses keloid fibrosis growth by inhibiting CyclinD1

Pei Liu1, Yaotian Hu, Lin Xia

  • 1Department of Plastic Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Journal of Biosciences
|April 30, 2020
PubMed

Insights

MicroRNA-4417 (miR-4417) is down-regulated in keloid fibrosis, suppressing cell proliferation and migration. Restoring miR-4417 levels may offer a new therapeutic strategy for keloid treatment.

Area of Science:

  • Biomedical research
  • Molecular biology
  • Dermatology

Background:

  • MicroRNAs (miRNAs) are crucial in keloid fibrosis development.
  • The specific role of miR-4417 in keloid fibrogenesis is largely unknown.
  • Previous studies linked miR-4417 to fibrogenesis in lung epithelial cells.

Purpose of the Study:

  • To investigate the role of miR-4417 in keloid fibrogenesis.
  • To elucidate the underlying molecular mechanisms involving miR-4417 and CyclinD1.
  • To assess the therapeutic potential of targeting the miR-4417/CyclinD1 axis in keloids.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for miR-4417 and CyclinD1 expression.
  • Cell Counting Kit (CCK) assay for cell proliferation.
  • Western blot and flow cytometry for cell apoptosis.
  • Transwell assays for cell migration and invasion.
  • Luciferase reporter assay to confirm miR-4417 and CyclinD1 interaction.

Main Results:

  • miR-4417 was significantly downregulated in keloid tissues and fibroblasts.
  • Overexpression of miR-4417 inhibited keloid fibroblast proliferation, migration, and invasion, while promoting apoptosis.
  • CyclinD1 expression was decreased in keloids and directly targeted by miR-4417.
  • miR-4417 negatively correlated with CyclinD1 expression in keloid tissues.
  • Modulating CyclinD1 mimicked or reversed the effects of miR-4417, confirming the miR-4417/CyclinD1 axis's role.

Conclusions:

  • miR-4417 acts as a tumor suppressor in keloid fibroblasts by inhibiting proliferation, migration, and invasion, and inducing apoptosis.
  • The miR-4417/CyclinD1 axis is a critical regulator of keloid fibroblast behavior.
  • miR-4417 and CyclinD1 represent potential therapeutic targets for keloid treatment.

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